The AmpliScribe™ T7 High Yield Transcription Kit is specially formulated to use high concentrations of NTPs, that can be inhibitory to other in vitro transcription systems. AmpliScribe High Yield Transcription reactions can produce >20-fold more full-length RNA transcript than conventional in vitro transcription reactions. For example, the AmpliScribe T7 Kit yields approximately 5-7.5 mg/ml of 1.4-kb full-length control RNA in 30-120 minutes, respectively, using 1 µg of template DNA (Fig. 1).
Note: Because of the very high concentration of NTPs used in AmpliScribe reactions, these kits are not recommended for preparing high specific-activity radiolabeled RNA probes.
|Figure 1. The AmpliScribe™ T7 High Yield Transcription Kit is specially formulated to consistently produce >20-fold more RNA than a conventional T7 in vitro transcription reaction.||Figure 2. An AmpliScribe™ T7 High Yield Transcription reaction produces full-length, long RNA transcripts. Aliquots from standard 20-µl AmpliScribe T7 High Yield Transcription reactions were loaded onto a 1% agarose-formaldehyde gel and stained with ethidium bromide. The size of the RNA transcripts and RNA yields (in parentheses) were: Lane 1, 3.0 kb (185 µg); lane 2, 4.0 kb (178 µg); lane 3, 5.0 kb (176 µg); lane 4, 6.0 kb (169 µg); lane M, RNA size marker.|