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Bst DNA Polymerase, Exonuclease Minus

  • High strand displacement activity.
  • Used in isothermal amplification and Next Generation Sequencing.
  • Ordering

  • Specifications

  • Manuals

  • Resources

Product Description Size Cat. No. Price Quantity BUY
Bst DNA Polymerase, Exonuclease Minus 8,000 U/ml  2,000 U 30027-1 $77.00
   10,000 (5 x 2,000 Units) 30027-2 $309.00
Bst DNA Polymerase, Exonuclease Minus 50,000 U/ml  10,000 Units 30028-1 $309.00
ORDER INFORMATION
Bst DNA Polymerase, Exonuclease Minus is available in two concentrations, 8,000 U/ml and 50,000 U/ml, in a storage buffer of 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, and 50% Glycerol. Also supplied is 10 X DNA Polymerase Buffer B, composed of 200 mM Tris-HCl pH 8.8, 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4,  and 1.0 % Triton X-100.

Storage Buffer: 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, and 50% Glycerol.
Purity: >99% pure by SDS-PAGE. No detectable DNA contamination. 10 µl of enzyme at 8 U/µl of the sample was tested for E. coli genomic DNA contamination by PCR amplifying with the E. coli 16S ribosomal primers.
Activity Determination: One unit catalyzes the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 65°C in 20 mM Tris-HCl pH 8.8, 10 mM (NH4)2SO4, 10 mM KCl, 2 mM MgSO4, 0.1 % Triton X-100, 30 nM M13mp18 ssDNA, 70 nM M13 sequencing primer(-47) 24 mer 200 µM dGTP, dATP, dTTP, dCTP (a mix of unlabeled and and 33P]dCTP), and 0.1 mg/ml BSA.
Absence of Endonuclease or Nicking Activity Incubation of 8 U and 50 U of Bst DNA Polymerase, Exonuclease Minus, with 1 µg of supercoiled pBR322 DNA for 16 hours at 37° and 65°C resulted in no detectable conversion to relaxed or linear forms by agarose gel electrophoresis.
Absence of Exonuclease Activity: Incubation of 8 U and 50 U of Bst DNA Polymerase, Exonuclease Minus, with 1 µg of HindIII-cut lambda DNA for 16 hours at 37° and 65°C resulted in no smearing of bands on agarose gels. Single stranded and double stranded exonuclease activities were tested by incubating 10 µl of enzyme at 8 U/µl with radiolabeled DNA substrate for one hour at 37° and 65°C, resulting in less than 0.1% release of TCA-soluble counts.

Document File Name Type
Manual MA073-Bst-DNA-Polymerase-8-U PDF
Manual MA074-Bst-DNA-Polymerase-50-U PDF

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