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CJ236 Electrocompetent Cells

High efficiency cells to create uracil-containing DNA for site-directed mutagenesis

  • Sole source of highly efficient Electrocompetent cells (1 × 109 cfu/µg)
  • ung- and dut- mutations to generate DNA with some uracil residues
  • Ideal genotype and efficiency for Kunkel directed mutagenesis method1.

1 Kunkel T.A. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection.  Proc. Natl. Acad. Sci. 52:488-492.

  • Ordering

  • Specifications

  • Manuals

  • Resources

Product Description Size Cat. No. Price Quantity BUY
CJ236 Electrocompetent Cells  12 rxns 60701-1 $169.00
   24 rxns 60701-2 $293.00
ORDER INFORMATION
Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

Genotype:
[F’ Tra+ Pil+ (CamR)] ung-1 relA1 dut-1 thi-1 spoT1 mcrA

Document File Name Type
Manual MA136-CJ236-ElectroCompetent-Cells PDF

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