Stability of GeCKO libraries in Endura cells. Lanes 1 and 2,Separate libraries were prepared in Lucigen Endura cells and grown at 37°C. Library 1 was re-amplified by growth at 32°C (Lane 3). Undesired recombination may become more pronounced at 37°C. Recombination becomes more pronounced by growth at 37°C (data not shown). Courtesy Dr. G. Winter, MIT Broad Inst.
Lucigen's Endura™ electrocompetent cells are an excellent choice for cloning and amplification of lentiviral vectors. They are highly recommended by researchers for lentiviral libraries of CRISPR clones (Clustered Regularly Interspaced Short Palindromic Repeats), particularly the GeCKO v2.0 plasmid libraries (REF) (See Figure). Learn more about CRISPR >
NOTE: Transformation of Endura Electrocompetent Cells with GeCKO lentiviral libraries may require extra Recovery Medium. The Zhang lab protocol (available from Addgene) recommends ~2 mL of Recovery Medium per transformation. Lucigen Endura kits provide 1 mL of Recovery Medium per transformation. Order extra Recovery Medium here!
REF: Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells. Shalem et al.
(2014). Science, Vol. 343 no. 6166 pp. 84-87.