CloneDirect™ Rapid Ligation Kit
- Ligation reaction is transformation-ready in minutes
- No DNA cleanup step required
- Highly reliable and reproducible results
The CloneDirect Rapid Ligation Kit is a simple, rapid, highly efficient alternative to standard ligation and transformation protocols. This kit eliminates the requirement for a post-ligation cleanup step before electroporation. A 15-minute heat denaturation at 70°C is all that is needed prior to cell transformation.
Figure 1. Ligation efficiencies measured by transformation. Ligation reactions were heat denatured at 70°C and used without additional cleanup to transform electrocompetent E. cloni® 10G cells. Transformants (in millions) are shown.
The CloneDirect Rapid Ligation Kit contains: 10X CloneDirect Buffer (contains ATP), CloneSmart® DNA Ligase, and complete protocols.