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Cloning Kits & Vectors

Lucigen has a wide variety of cloning systems, including novel transcription-free cloning vectors, that improve cloning yields, reduce cloning bias, and improve sequencing throughput. For cloning the most difficult sequences, the BigEasy® v2.0 Linear Cloning System offers unmatched stability. Most Lucigen cloning vectors are pre-cut, dephosphorylated, and ready-to-use.

Go directly to Vector Sequences >

Also see Protein Expression >

General Cloning

CloneSmart® Cloning Kits (pSMART® Vectors) » Stably clone any insert in transcription-free, blunt vectors for general cloning. Choice of antibiotic resistance and copy number.

GC Cloning and Amplification (pGC™ Blue Vector) » Clone PCR products with optional blue/white screening; includes T7 & SP6 RNA polymerase promoters.

Difficult Cloning or BAC/Fosmid Cloning

BigEasy® v2.0 Linear Cloning System (pJAZZ® Vectors) » Clone the unclonable, including large or highly repetitive DNA, solely available from Lucigen.

CopyControl™ Fosmid Library Production Kits » Efficiently clone any insert up to 40 kb with a complete kit, including packaging extracts.

MaxPlax™ Lambda Packaging Extracts » Package fosmid, cosmid or lambda DNA to generate genomic libraries.

CopyRight® v2.0 BAC Cloning Kits (pSMART® BAC and pEZ™ BAC) » Efficiently clone any insert 200kb.

CopyRight® v2.0 Fosmid Cloning Kits (pSMART® FOS) » Efficiently clone any insert 35-45kb.

Accessory Kits for Cloning

DNATerminator® End Repair Kit » Repair sheared or restriction-digested DNA to create blunt, 5' phosphorylated ends.

CloneDirect™ Rapid Ligation Kit » Proceed from ligation reaction to transformation in minutes.

Gel-Ready™ DNA Ladders » Bench-top stable markers — always ready to use.

End-It™ DNA End-Repair Kit » Prepare blunt, phosphorylated DNA ends from PCR product or sheared DNA.

GELase™ Agarose Gel-Digesting Preparation » Recover DNA and RNA from low-melting point agarose gels.

Fast-Link™ DNA Ligation Kit » Ligate DNA for cloning applications.

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