Lucigen's CopyRight® v2.0 BAC cloning kits with pSMART® BAC (Figure1) or pEZ BAC (Figure 2) vector and BAC-Optimized Replicator™ v2.0 Electrocompetent Cells result in unprecedented accuracy, reliability, and efficiency in BAC cloning and library construction. These vectors incorporate the CloneSmart® transcription-free technology, which minimizes cloning bias and enables cloning of otherwise toxic genes, along with inducible copy number amplification (20-50X) for high DNA yields and a lacZ-sacB stuffer fragment that eliminates uncut vector from recombinants, resulting in zero background. Unlike other BAC cloning systems, Lucigen's CloneSmart technology assures that even "unclonable" inserts are stably maintained. Sequences are not lost or rearranged in the cloning process. CopyRight Kits offer superior performance for BAC cloning at a much better price than other kits. Figure 3 shows an example of a Random Shear BAC library generated with pSMART BAC.
Figure 1. CopyRight vector pSMART BAC. ori2, repE, IncC - single-copy origin of replication; oriV - inducible origin of replication; par A,B,C - partition genes; Cm^r - chloramphenicol-resistance gene; cosN - lambda packaging signal; T - CloneSmart transcription terminators; sacB -sucrase gene. Approximate positions of sequencing primers and transcription terminators are indicated.
Figure 2. CopyRight vector pEZ BAC. ori2, repE, IncC, single-copy origin of replication; oriV - inducible origin of replication; par A,B,C, partition genes; Cm^r - chloramphenicol-resistance gene; cosN - lambda packaging signal; lacZ - alpha peptide of the beta galactosidase gene. Approximate positions of sequencing primers and transcription terminators are indicated.
Figure 3. Arabidopsis Random Shear BAC Library in the pSMART BAC vector. 7,680 clones were obtained, with an average insert size of 105 kb and ~5X genome coverage.
Efficiently obtain your clone or create your library—the CopyRight v2.0 Cloning Kits eliminate tedious vector and competent cell preparation, as well as time-consuming QC experiments. Kits include optimized reagents, ligation-ready vector, highly efficient electrocompetent cells, detailed instructions, and a trouble-shooting guide.
|Citations||CopyRight® v2.0 BAC Cloning Kits (pSMART® BAC and pEZ™ BAC) - Citations||LINK|
|SDS||SDS032- 60210-1_60210-2 BAC-Optimized Replicator Electrocompetent Cells.pdf|
|SDS||SDS074 42009-1_42009-2 CopyRight v2.0 pEZ BAC Blunt Cloning Kit with BAC-Optimized Replicator v.2.0 Electrocompetent Cells.pdf|
|SDS||SDS075 42016-1 CopyRight v2.0 pEZ BAC Blunt Cloning Kit.pdf|