The cookie settings on this website are adjusted to allow all cookies so that you have the very best experience. If you continue without changing your cookie settings, we'll assume that you are happy to receive all cookies on our website. However, if you would like to, you can change your settings at any time using the Change cookie settings link in the Special menu.
Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required. These cells provide solutions for a wide range of applications at economical prices.
E. cloni 10G Competent Cells. Routine cloning, subcloning, and plasmid isolation with or without blue/white screening.
Choice of efficiency:
E. cloni 10G Chemically Competent Cells (> 1 × 109 cfu/µg pUC DNA; (> 1 × 108 in 96-well plates). Highly efficient competent cells for routine cloning applications. Available in one transformation per tube (SOLOs) or two transformations per tube (DUOs). Also available in 96-well plates (which can be divided into 24-well seqments) for higher throughput.
E. cloni 10G Chemically Competent Cells, Subcloning Grade (> 1 × 106 cfu/µg pUC DNA). The best value available anywhere for simple cloning and plasmid propagation.
Custom Competent Cells
Bulk Lucigen cells or your strain of E. coli, made to the highest possible transfection efficiency with custom dispensing! See Custom Competent Cells page for detailed information.
Table 1. E. cloni Competent Cells for Cloning
E. cloni Cell Lines
Transformation Efficiency (cfu/µg pUC DNA)
Cloning Methylated DNA
BAC, Cosmid Cloning
10G Chemically Competent
>1 × 109
YES: without IPTG induction
10G Chemically Competent Subcloning Grade
>1 × 106
YES: without IPTG induction
E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA
E. cloni 10G Chemically Competent Cells > 1 × 109 cfu/µg pUC DNA; 96-well format > 1 × 108 in 96-well plates
Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), Subcloning Grade (12 transformations per tube), or microplates as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
PLEASE NOTE: Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all Lucigen Competent Cells. Please contact Lucigen.