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E. cloni® 10G Chemically Competent Cells

  • Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.)
  • Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable.
  • Available in a range of transformation efficiencies (1 × 106 to 1 x 109cfu/µg).
  • Convenient packaging options.
  • High efficiency cells result in high colony counts
  • Endorsed by SGI-DNA for Gibson Assembly® applications

Looking for electrocompetent version?

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  • Ordering

  • Specifications

  • Manuals

  • Resources

Filter products by:
Size:
[i] Transformations/Tube:
Efficiency:
Product Description Size Cat. No. Price Quantity BUY
E. cloni 10G Chemically Competent Cells > 1 × 10^9 cfu/µg  12 rxns (DUOs) 60107-1 $117.00
   24 rxns (DUOs) 60107-2 $205.00
   48 rxns (DUOs) 60107-3 $339.00
   96 rxns (DUOs) 60107-4 $603.00
E. cloni 10G Chemically Competent Cells > 1 × 10^9 cfu/µg   12 rxns (SOLOs) 60106-1 $135.00
   24 rxns (SOLOs) 60106-2 $235.00
   48 rxns (SOLOs) 60106-3 $391.00
E. cloni 10G Chemically Competent Cells (Subcloning Grade) > 1 × 10^6 cfu/µg  48 rxns 60108-1 $67.00
   96 rxns 60108-2 $131.00
E. cloni 10G Chemically Competent Cells > 1 × 10^8 in 96-well plates  1 plate (SOLOs) 60096-1 $424.00
   4 plates (SOLOs) 60096-4 $1492.00
ORDER INFORMATION

Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), Subcloning Grade (12 transformations per tube), or microplates as indicated. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

PLEASE NOTE: Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all Lucigen Competent Cells. Please contact Lucigen.

Genotypes

E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697galgalrpsnupG λ- tonA (StrR)

Document File Name Type
Manual MA010-Ecloni-10G-Chem-Comp PDF
Manual MA050-Ecloni-96-well-10G-ChemComp PDF

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“Lucigen’s cells ROCK! Few, if any, companies or individual laboratories make cells with as high efficiencies.” - Dr. Douglas Crawford, University of Miami, Miami, FL


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