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E. cloni® 10G and 10GF' Electrocompetent Cells

  • Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.)
  • Optimized genetics for high yields:  phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable.
  • Available in a range of high transformation efficiencies (5 × 109 to 4 × 1010 cfu/µg).
  • Convenient packaging options.

Looking for chemically competent version?

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  • Ordering

  • Specifications

  • Manuals

  • Resources

Filter products by:
Size:
[i] Transformations/Tube:
Efficiency:
Product Description Size Cat. No. Price Quantity BUY
E. cloni 10GF' ELITE Electrocompetent Cells, > 2 × 10^10 cfu/µg  12 rxns (DUOs) 60061-1 $161.00
   24 rxns (DUOs) 60061-2 $279.00
E. cloni 10G CLASSIC Electrocompetent Cells > 5 × 10^9 cfu/µg  24 rxns (SixPacks) 60117-1 $185.00
   48 rxns (SixPacks) 60117-2 $331.00
E. cloni 10G ELITE Electrocompetent Cells > 2 × 10^10 cfu/µg  12 rxns (SOLOs) 60051-1 $183.00
   24 rxns (SOLOs) 60051-2 $318.00
   4 rxns 60052-0 $0.00
   12 rxns (DUOs) 60052-1 $159.00
   24 rxns (DUOs) 60052-2 $276.00
   24 rxns (SixPacks) 60052-3 $212.00
   48 rxns (SixPacks) 60052-4 $352.00
E. cloni 10G SUPREME Electrocompetent Cells > 4 × 10^10 cfu/µg   12 rxns (SOLOs) 60081-1 $288.00
   24 rxns (SOLOs) 60081-2 $510.00
E. cloni 10G SUPREME Electrocompetent Cells >4 × 10^10 cfu/µg   12 rxns (DUOs) 60080-1 $252.00
   24 rxns (DUOs) 60080-2 $464.00
ORDER INFORMATION

Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), or SixPacks (6 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

PLEASE NOTE: Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all Lucigen Competent Cells. Please contact Lucigen.

Genotypes

E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697galgalrpsnupG λ- tonA (StrR)

E. cloni 10GF´: [F´ pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galgalrpsnupG λ- tonA (StrR)


Efficiencies

E. cloni 10G SUPREME Electrocompetent Cells > 4 × 1010 cfu/µg pUC DNA

E. cloni 10G & 10GF´ ELITE Electrocompetent Cells > 2 × 1010 cfu/µg pUC DNA

E. cloni 10G CLASSIC Electrocompetent Cells > 5 × 109 cfu/µg pUC DNA 

Document File Name Type
Manual MA009-Ecloni-10G-10GF'-Electrocompetent PDF

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“Lucigen’s cells ROCK! Few, if any, companies or individual laboratories make cells with as high efficiencies.” - Dr. Douglas Crawford, University of Miami, Miami, FL


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