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EconoTaq® DNA Polymerase

  • Great Taq Polymerase performance at a low price.
  • Choice of reaction buffers, with or without MgCl2.
  • Non-proofreading Polymerase

Free Sample Available Free Sample Available

 

  • Ordering

  • Specifications

  • Manuals

  • Resources

Product Description Size Cat. No. Price Quantity BUY
EconoTaq DNA Polymerase (with Mg++)  1,000 U 30031-1 $96.00
   5,000 U (5 x 1,000 U) 30031-2 $456.00
   10,000 U (10 X 1,000 U) 30031-3 $888.00
EconoTaq DNA Polymerase (separate Mg++)  1,000 U 30032-1 $96.00
   5,000 U (5 x 1,000 U) 30032-2 $456.00
   10,000 U (10 X 1,000 U) 30032-3 $888.00
ORDER INFORMATION
EconoTaq DNA Polymerase is provided with a choice of 10X Reaction Buffer with Mg++ (“with Mg++”); or 10X Reaction Buffer without Mg++ and a separate tube of 25 mM MgCl2 (“separate Mg++”). Please inquire for Bulk pricing!

Concentration: 5 units/µl. One unit catalyzes the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 70°C in 50 mM Tris-HCl (pH 9.0), 50 mM NaCl, 5 mM MgCl2, 200 µM dGTP, dATP, dTTP dCTP (a mix of unlabeled and [33P] dCTP), 10 µg of activated calf thymus DNA, and 0.1 mg/ml BSA.

Storage Buffer: 10 mM Tris-HCl (pH 7.5), 100 mM KCl, 0.1% Triton X-100, 0.1 mM EDTA, 1mM DTT, and 50% glycerol.

10X Reaction Buffer:100mM Tris-HCl (pH 9.0), 500 mM KCl, 1% Triton X-100, and with or without 15mM MgCl2.

PCR Activity: EconoTaq DNA Polymerase is tested in DNA amplification using a variety of templates and primers.

Activity Determination: One unit of EconoTaq DNA Polymerase catalyzes the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 70°C in 50 mM Tris-HCl (pH 9.0), 50 mM NaCl, 5 mM MgCl2, 200 µM dGTP, dATP, dTTP, dCTP (a mix of unlabeled and [33P]dCTP), 10 µg Activated Calf Thymus DNA, and 0.1 mg/ml BSA.

Absence of Endonuclease or Nicking Activity: Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of supercoiled pBR322 DNA for 16 hours at 70°C results in no detectable conversion to relaxed or linear forms detectable by agarose gel electrophoresis.

Absence of Exonuclease Activity: Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of HindIII-cut lambda DNA for 16 hours at 70°C resulted in no smearing of bands on agarose gels.

Purity: EconoTaq DNA Polymerase is >99% pure as determined by SDS PAGE. There is no detectable DNA contamination.

Document File Name Type
Manual MA021_EconoTaq PDF
Manual MA029_EconoTaq_woMg PDF

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"The EconoTaq products are very reliable as well as cost effective."
Adrienne Moran Lauter,
Iowa State University

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