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EconoTaq® PLUS and EconoTaq PLUS GREEN 2X Master Mixes

  • Outstanding performance and value are perfect for routine PCR.
  • Can be cycled up to 98°C to amplify the most challenging GC-rich templates others cannot.
  • Ready-to-use PCR reaction master mixes, contain dNTPs and PCR Enhancer.
  • Non-proofreading polymerase
  • EconoTaq PLUS GREEN contains tracking dyes for gel electrophoresis

Free Sample Available Free Sample Available

 

  • Ordering

  • Specifications

  • Manuals

  • Resources

Product Description Size Cat. No. Price Quantity BUY
EconoTaq PLUS GREEN 2X Master Mix  10 rxns 30033-0 $0.00
   500 rxns 30033-1 $162.00
   1,000 rxns 30033-2 $272.00
EconoTaq PLUS 2X Master Mix  50 rxns 30035-0 $0.00
   500 rxns 30035-1 $162.00
   1,000 rxns 30035-2 $272.00
ORDER INFORMATION
Use EconoTaq PLUS GREEN 2X Master Mix when the PCR products will be analyzed by agarose gel electrophoresis and ethidium bromide staining. Use EconoTaq PLUS 2X Master Mix when the PCR products will be analyzed by absorbance or fluorescence excitation, to avoid possible interference by the dyes. EconoTaq PLUS GREEN & EconoTaq PLUS prices are based on a 50 µl PCR reaction.

PCR Activity: EconoTaq PLUS GREEN & EconoTaq PLUS 2X Master Mixes are tested in DNA amplification using a variety of templates and primers.

Activity Determination: One unit of EconoTaq DNA Polymerase catalyzes the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 70°C in 50 mM Tris-HCl (pH 9.0), 50 mM NaCl, 5 mM MgCl2, 200 µM dGTP, dATP, dTTP, dCTP (a mix of unlabeled and [33P]dCTP), 10 µg Activated Calf Thymus DNA, and 0.1 mg/ml BSA.

Absence of Endonuclease or Nicking Activity: Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of supercoiled pBR322 DNA for 16 hours at 70°C results in no detectable conversion to relaxed or linear forms detectable by agarose gel electrophoresis.

Absence of Exonuclease Activity: Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of HindIII-cut lambda DNA for 16 hours at 70°C resulted in no smearing of bands on agarose gels.

Purity: EconoTaq DNA Polymerase is >99% pure as determined by SDS PAGE. There is no detectable DNA contamination.The nucleotides in the Master Mix are certified free of nucleases and phosphatases.

Document File Name Type
Manual MA037-EconoTaq-PLUSGREEN PDF
Manual MA038-EconoTaq-PLUS PDF

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“I love this product! I'm completely switching over - it's fool-proof.”
-Leesa LaFever, Vanderbilt University Medical Center, Nashville, TN

“I will continue to use EconoTaq PLUS GREEN 2X Master Mix for all of my genotyping protocols. Its relative simplicity is superb, and the price can’t be beaten!”
-Erin Plisch, Dept. of Pathobiological Sciences, University of Wisconsin-Madison.

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