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Endura™ Competent Cells

Clone repetitive sequences and lentiviral libraries

  • Stabilize direct repeats and create lentiviral constructs
  • Generate lentiviral guide RNA libraries
  • Recommended in CRISPR GeCKO library protocols
  • Choose electrocompetent or chemically competent cells
  • Highest efficiency commercially available cells for lentiviral cloning:  over 1 × 107 cfu/µg (chem) or 1 × 1010 cfu/µg (electro)

Transformation of Endura Electrocompetent Cells with GeCKO lentiviral libraries may require extra Recovery Medium.  Order extra Recovery Medium below.

Free Sample Available Free Sample Available

  • Ordering

  • Specifications

  • Manuals

  • Resources

Product Description Size Cat. No. Price Quantity BUY
Endura™ Chemically Competent Cells  12 rxns (DUO) 60240-1 $151.00
   24 rxns (DUO) 60240-2 $265.00
   12 rxns (SOLO) 60241-1 $167.00
   24 rxns (SOLO) 60241-2 $290.00
Endura™ ElectroCompetent Cells  4 rxns (DUO) 60242-0 $0.00
   12 rxns (DUO) 60242-1 $180.00
   24 rxns (DUO) 60242-2 $317.00
Recovery Medium  8 × 12 ml 80026-1 $109.00
ORDER INFORMATION

Endura™ Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube) or DUOs (2 transformations per tube) as indicated. Endura Electrocompetent cells are provided in DUOs format only. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

PLEASE NOTE: Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all Lucigen Competent Cells. Please contact Lucigen.

NOTE:  Transformation of Endura Electrocompetent Cells with GeCKO lentiviral libraries may require extra Recovery Medium.  The Zhang lab protocol (available from Addgene) recommends ~2 mL of Recovery Medium per transformation.  Lucigen Endura kits provide 1 mL of Recovery Medium per transformation.  Order extra Recovery Medium above or visit the Recovery Medium product page here!

Genotype:

recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)
Document File Name Type
Manual MA133-Endura-Competent-Cells PDF

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