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Enzymes

Lucigen provides a wide range of enzymes to for molecular biology and Next Gen Sequencing applications. Selection Guide Enzymes

  • Enzymes selected to meet your DNA and RNA synthesis needs

Also see enzymes for PCR & Amplification including Bst DNA Polymerase.

Modifying Enzymes

NxGen® T4 DNA Ligase » Reliably join double-stranded nucleic acid chains.

ATP Solution » Stable ATP solution for molecular biology applications.

Ampligase® Thermostable DNA Ligase » A thermostable DNA ligase for high temperature ligation-based applications.

CircLigase™ ssDNA Ligase » A ssDNA ligase well-suited to high temperature ligation-based applications.

CircLigase™ II ssDNA Ligase » A non-catalytic, thermostable ssDNA ligase.

T4 RNA Ligase 2, Deletion Mutant » An improved T4 RNA Ligase for addition of single-stranded adenlylated DNA or RNA oligos to RNA targets.

T4 Polynucleotide Kinase, Cloned » Phosphorylate non-phosphorylated DNA and RNA molecules for use in wide array of downstream applications.

RNA 5´ Polyphosphatase » Dephosphorylate tri- and di-phosphorylated RNA molecules while avoiding 5'-capped and mono-phosphorylated RNAs

T4 Polynucleotide Kinase » Add 5’ phosphate, with or without radiolabel, to end of single- or double-stranded DNA.

DNA Nucleases

RNase-Free DNase I » DNase I with no contaminating RNase activity

Baseline-ZERO™ Dnase » Digest large or small DNA molecules better than standard Dnase I

Plasmid-Safe™ ATP-Dependent Dnase » Digest unwanted contaminating linear dsDNA without harming circular dsDNA such as plasmids and fosmids.

Exonuclease VII » ssDNA exonuclease with both 5´ to 3´ and 3´ to 5´ exonuclease activities.

Exonuclease III, E. coli » 3' exonuclease that is active on nicks, blunt ends and 3'-recessed ends in dsDNA.

Rec J Exonuclease » 5' to 3' digestion of single-stranded DNA with this magnesium-dependent exonuclease.

Exonuclease I, E. coli » Digest single-stranded DNA from the 3' to 5' end, but not double-stranded or dsDNA with short 3' overhangs.

RNA Nucleases

Hybridase™ Thermostable RNase H » A thermostable, RNase H for the degradation of RNA in RNA:DNA hybrids.

RNase A » Efficiently degrade unwanted RNA in DNA preparations and other molecular biology samples/reactions.

RNase I, E. coli » Completely digest single-stranded RNA with this heat labile, non-sequence specific RNase

RNase R » A 3´ to 5´ exoribonuclease that digests all linear RNAs except lariat or circular RNA structures making it well-suited to RNA splicing and other research applications.

Terminator™ 5´-Phosphate-Dependent Exonuclease » A 5´ to 3´ riboexonuclease that digests RNA with 5´-monophosphate ends but not RNAs with 5´-triphosphate, 5´-cap or 5´-hydroxyl groups.

DNA and RNA Nucleases

OmniCleave™ Endonuclease » Digests all forms of unwanted DNA and RNA in multiple samples types.

Polymerases

NxGen® phi29 DNA Polymerase » Excellent strand displacement, fidelity and speed.

NxGen® T7 RNA Polymerase » Reliable transcription.

T7 R&DNA™ Polymerase » A mutatated T7 RNA polymerase that incorporates dNTPs as well as normal NTPs into in vitro transcribed RNA

Exo-Minus Klenow DNA Polymerase (D355A, E357A) » A Klenow DNA polymerase lacking all 5' to 3' and 3' to 5' exonuclease activity.

Poly(A) Polymerase Tailing Kit » Add poly(A) tails to the 3' ends of any RNA molecules with this robust kit.

T7 RNA Polymerase » Produce high yields of in vitro transcribed RNA from any dsDNA template containing a wild type T7 RNA polymerase promoter.

T4 DNA Polymerase, Exonuclease Minus » Increase the DNA sequencing read-lengths.

Reverse Transcriptase Enzymes

EpiScript™ RNase H- Reverse Transcriptase » Increase specificity with an RT stable to 55°C.

MMLV High Performance Reverse Transcriptase » Produce more full-length, first strand cDNA

NxGen® M-MuLV Reverse Transcriptase » Outstanding cDNA synthesis.

Sygnis SunScript™ Reverse Transcriptase RNase H- » A revolutionary novel reverse transcriptase with improved properties related to thermostability, efficiency and synthesis of long transcripts.

RNase Inhibitors

NxGen® RNase Inhibitor » Eliminate RNase activity.

RiboGuard™ RNase Inhibitor » Inhibit RNase activity

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