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Expresso® T7 Cloning and Expression System

Clone and express even difficult proteins with the fastest and easiest directional PCR-based cloning system available.
  • Save time! Enzyme-free cloning in seconds with cloning-ready vector and cells.
  • High efficiency: ›90% recombinants.
  • Tightly-controlled expression of N- or C-terminal 6xHis tagged proteins.

Also available with cleavable SUMO Solubility Tag

  • Ordering

  • Specifications

  • Manuals

  • Resources

Product Description Size Cat. No. Price Quantity BUY
Expresso® T7 Cloning and Expression System, N-His  5 rxns 49001-1 $200.00
   10 rxns 49001-2 $366.00
Expresso® T7 Cloning and Expression System, C-His  5 rxns 49002-1 $200.00
   10 rxns 49002-2 $366.00
Expresso® T7 Cloning and Expression System, N/C-His Combo, 5 of each of N-His and C-His  10 rxns 49000-1 $366.00
Expresso® T7 SUMO Cloning and Expression System  5 rxns 49003-1 $277.00
   10 rxns 49003-2 $496.00
HI-Control™ 10G Chemically Competent Cells (SOLOs)  12 rxns (SOLOs) 60110-1 $162.00
HI-Control™ BL21(DE3) Chemically Competent Cells (SOLOs)   12 rxns (SOLOs) 60435-1 $162.00
SUMO Express Protease  200 U 30801-2 $228.00
ORDER INFORMATION
The Expresso T7 Cloning & Expression System contains pre-processed pETite® N-His and/or pETite C-His Vector DNA, HI-Control™ 10G Chemically Competent Cells for cloning, and HI-Control BL21(DE3) Chemically Competent Cells for protein expression. Also included are N-His and/or C-His Positive Control Insert DNAs, and transformation positive control pUC DNA.

The Expresso T7 SUMO Cloning and Expression System contains pre-processed pETite® N-His SUMO Vector DNA, HI-Control™ 10G Chemically Competent Cells for cloning, and HI Control BL21(DE3) Chemically Competent Cells for protein expression. Also included are SUMO Positive Control C Insert DNA, transformation positive control pUC DNA, SUMO Express Protease, SUMO Cleavage Control Protein, and forward and reverse PCR primers to confirm clones.

Please note that the SUMO Protease and SUMO Fusion tag are modified from the original native proteins. Native or non-modified control proteins or proteases will not be compatible. For any questions about the SUMO tag or SUMO Protease and associated uses, please contact Lucigen Technical Service.

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"I was extremely pleased with the Expresso system - I got my clones very easily, in one quick try, and have highly recommended the product to my colleagues here at MIT."
– Parson’s lab, MIT

"Love this product - takes the hassle out of cloning for protein expression in E.coli. Fast, more reliable than traditional methods, and very easy.
Samantha Zelin, Agrivida

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