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Expresso® Rhamnose Cloning & Protein Expression System

Clone a PCR amplified gene in an effortless afternoon, and express recombinant protein the next day.

  • Five-second, directional, enzyme-free PCR cloning! 90% recombinants!
  • Single competent cell host strain for both cloning and expression.
  • Tighter expression control with tunable Rhamnose (rhaPBAD) promoter.
  • High throughput format friendly with autoinduction reagents.

Also available with cleavable SUMO Solubility Tag

  • Ordering

  • Specifications

  • Manuals

  • Resources

Product Description Size Cat. No. Price Quantity BUY
Expresso® Rhamnose Cloning and Expression System, C-His  5 rxns 49012-1 $200.00
   10 rxns 49012-2 $366.00
Expresso® Rhamnose Cloning and Expression System, N-His   5 rxns 49011-1 $200.00
   10 rxns 49011-2 $366.00
Expresso® Rhamnose Cloning and Expression System, N/C-His Combo, 5 reaction of each of N-His and C-His  10 rxns 49010-1 $366.00
Expresso® Rhamnose SUMO Cloning and Expression System  5 rxns 49013-1 $277.00
   10 rxns 49013-2 $496.00
Glucose Solution, 15% w/v  5 x 1.25 ml 49022-1 $57.00
Rhamnose Solution, 20% w/v  5 x 1.25 ml 49021-1 $57.00
SUMO Express Protease  200 U 30801-2 $228.00
ORDER INFORMATION

The Expresso Rhamnose Cloning & Expression System contains pre-processed pRham N-His and/or pRham C-His Vector DNA, single-transformation E. cloni 10G Chemically Competent Cells (SOLOs), and the auto-induction reagents 20% Rhamnose solution and 15% Glucose solution. Also included are N-His and/or C-His Positive Control Insert DNAs, transformation positive control pUC DNA, and forward and reverse PCR primers to confirm clones.

The Expresso Rhamnose SUMO Cloning and Expression System contains pre-processed pRham N-His SUMO Vector DNA, single-transformation E. cloni 10G Chemically Competent Cells (SOLOs), and the auto-induction reagents 20% Rhamnose solution and 15% Glucose solution. Also included are SUMO Positive Control C Insert DNA, transformation positive control pUC DNA, SUMO Express Protease, SUMO Cleavage Control Protein, and forward and reverse PCR primers to confirm clones.

Please note that the SUMO Protease and SUMO Fusion tag are modified from the original native proteins. Native or non-modified control proteins or proteases will not be compatible. For any questions about the SUMO tag or SUMO Protease and associated uses, please contact Lucigen Technical Service.

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