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Expresso® SUMO Cloning and Expression Systems

Soluble protein has never been this fast and easy!

  • Enzyme-free directional PCR cloning in seconds!
  • Save days of effort with ready-to-use vectors and competent cells - NO ligation step.
  • Tightly-controlled expression of N-terminal 6xHis-tagged proteins with cleavable SUMO solubility tag.
  • Systems available with expression under the control of T7 or tunable Rhamnose promoters.
  • Ordering

  • Specifications

  • Manuals

  • Resources

Product Description Size Cat. No. Price Quantity BUY
Expresso® Rhamnose SUMO Cloning and Expression System  5 rxns 49013-1 $277.00
   10 rxns 49013-2 $496.00
Expresso® T7 SUMO Cloning and Expression System  5 rxns 49003-1 $277.00
   10 rxns 49003-2 $496.00
Arabinose Solution, 1000X  5 x 1.0 ml 49023-1 $55.00
Glucose Solution, 15% w/v  5 x 1.25 ml 49022-1 $57.00
HI-Control™ 10G Chemically Competent Cells (SOLOs)  12 rxns (SOLOs) 60110-1 $162.00
HI-Control™ BL21(DE3) Chemically Competent Cells (SOLOs)   12 rxns (SOLOs) 60435-1 $162.00
Rhamnose Solution, 20% w/v  5 x 1.25 ml 49021-1 $57.00
SUMO Express Protease  200 U 30801-2 $228.00
ORDER INFORMATION

The Expresso T7 SUMO Cloning and Expression System contains pre-processed pETite® N-His SUMO Vector DNA, HI-Control™ 10G Chemically Competent Cells for cloning, and HI Control BL21(DE3) Chemically Competent Cells for protein expression. Also included are SUMO Positive Control C Insert DNA, transformation positive control pUC DNA, SUMO Express Protease, SUMO Cleavage Control Protein, and forward and reverse PCR primers to confirm clones.

The Expresso Rhamnose SUMO Cloning and Expression System contains pre-processed pRham N-His SUMO Vector DNA, single-transformation E. cloni 10G Chemically Competent Cells (SOLOs), and the auto-induction reagents 20% Rhamnose solution and 15% Glucose solution. Also included are SUMO Positive Control C Insert DNA, transformation positive control pUC DNA, SUMO Express Protease, SUMO Cleavage Control Protein, and forward and reverse PCR primers to confirm clones.

Please note that the SUMO Protease and SUMO Fusion tag are modified from the original native proteins. Native or non-modified control proteins or proteases will not be compatible. For any questions about the SUMO tag or SUMO Protease and associated uses, please contact Lucigen Technical Service. Lucigen SUMO tags are compatible with SUMO Star protease from LifeSensors, but not SUMO Protease I.

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“Very easy cloning, and increased total soluble protein expression!”

-Chris Lucchesi, SUNY Upstate


"Love this product - takes the hassle out of cloning for protein expression in E.coli. Fast, more reliable than traditional methods, and very easy.
Samantha Zelin, Agrivida

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