The OmniAmp® polymerase is the only enzyme capable of Loop-mediated Isothermal Amplification (LAMP) from both RNA and DNA samples. The enzyme has a temperature optimum of 72°C, allowing increased specificity and flexibility as compared with Bst Exo Minus or two-step processes. The native reverse transcriptase activity of this enzyme means no longer adding a separate reverse transcriptase for RNA targets.
Flexibility: The enzyme comes with 10X Polymerase Buffer C, Magnesium Chloride, and Betaine. The buffer is designed to allow for a wide range of additive concentrations. Easily optimize your reaction with the instructions provided in the user manual.
Reliability: Achieve faster amplification at higher temperatures without sacrificing sensitivity. The enzyme has high target specificity and low background. Every batch is quality tested to ensure no unwanted nuclease activity will interfere with your sensitive applications.
Primer design: A robust LAMP reaction depends on proper design of the amplification primers. Please follow this PDF guide to properly use the PrimerExplorer software. The use of six primers is optimal; reactions are significantly slower and detection is less sensitive if only four primers are used.
Fig 1. RNA LAMP reaction
|Fig. 2. Quantitation of LAMP Amplification from DNA Target |
Quantitative results of LAMP amplification of E. ictaluri DNA over several orders of magnitude of target concentration. Color key: 1:10 = Red, 1:100 = Blue, 1:1,000 = Brown, 1:10,000 = Green, 1:100,000 = Pink, 1:1,000,000 = Light Blue. 1:10,000,000 dilution (yellow) and NTC's (Black) showed no amplification. The cycler was programmed to read amplification in 30-second intervals (cycles).
Fig. 3. DNA LAMP amplification
Licensing information: Lucigen is a fully licensed provider of LAMP reagents for research use. Patents WO 00/28082, WO 01/34790, and WO 01/77317 regarding the LAMP method are owned by the Eiken Chemical Co. Ltd. OmniAmp™ and Bst Polymerase, Exonuclease minus are sold by Lucigen under license for use in LAMP for research use only. The products may not be used for LAMP-based human or diagnostic purposes without obtaining a license from Eiken. US Patent 8093030 for OmniAmp DNA Polymerase is owned by Lucigen Corp.
It is the sole responsibility of the buyer to ensure that use of the product does not infringe the patent rights of third parties. If the purchaser is not willing to accept these use limitations, Lucigen Corporation is willing to accept return of the product for a full refund.
Absence of Endonuclease or Nicking Activity: Incubation of OmniAmp™ DNA Polymerase with 1 µg of supercoiled pBR322 DNA for 16 hours at 70°C results in no detectable conversion to relaxed or linear forms detectable by agarose gel electrophoresis.
Absence of Exonuclease Activity: Incubation of of OmniAmp DNA Polymerase with 1 µg of HindIII-cut lambda DNA for 16 hours at 70°C resulted in no smearing of bands on agarose gels.
Purity: OmniAmp DNA Polymerase is >99% pure as determined by SDS-PAGE.
Exogenous DNA: There is no detectable DNA contamination.