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Sygnis TruePrime™ WGA Kit

Whole genome amplification (WGA) with less bias making this kit ideally suited for next generation sequencing applications.

  • High sensitivity: Typical yield is greater than 5 µg when starting with 1 ng of genomic DNA.
  • Reduced amplification bias and no primer artefacts: Primer-free amplification method utilizes a combination of TthPrimPol Primase (to synthesize primers) and Phi29 DNA polymerase.
  • Well suited for next generation sequencing: Tested in Illumina and Ion Torrent NGS workflows.
  • Easy and reliable
  • Insensitive to external DNA contamination
  • Ordering

  • Specifications

  • Manuals

  • Resources

Product Description Size Cat. No. Price Quantity BUY
Sygnis TruePrime™ WGA Kit  25 rxn SYG370025 $248.00
   100 rxn SYG380100 $675.00
ORDER INFORMATION

Each TruePrime™ WGA Kit contains: Buffer D, Buffer N, Reaction Buffer, dNTPs, Water, Enzyme 1 and Enzyme 2. The complete manual is available under the Manual tab. Lucigen is an authorized distributor of Sygnis products in the US.

TruePrime™ WGA Kit is intended for molecular biology use only and in vitro use only. This product is not intended for diagnosis, prevention or treatment of a disease in human beings or animals.

Technical Specifications:
Typical DNA yields from a TruePrime™ WGA kit reaction are about 5 µg per 50 µL reaction when starting with 1 ng of DNA. Yields and kinetics will vary if crude or un-quantified samples are amplified. Reactions without input DNA (no template controls) do not produce any amplification product during 3 hour reactions. Mean product length is greater than 10 kb. Store amplified DNA at 4°C for short-term or at -20°C for long-term storage.

Quality Control:
Each batch of TruePrime WGA Kits is tested against predetermined specifications to ensure consistent product quality. Enzymes used in the kit have been tested separately to ensure adherence to specifications.

Shipping and Storage:
Kits are shipped on dry ice. Upon receipt, the kit should be stored immediately at -20°C in a non-frost-free (constant-temperature) freezer. When stored under these conditions and handled correctly, the products can be kept at least six months after shipping without showing any reduction in performance. For longer periods of time, store the kit at -80°C.

Document File Name Type
Manual Sygnis-Handbook_TruePrime_WGA_Kit PDF
Document File Name Type
SDS Sygnis-TruePrime™-WGA-kit-MSDS.pdf PDF

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