T4 DNA Polymerase, Exonuclease Minus is a DNA-dependent DNA polymerase which catalyzes the 5’→3’ synthesis of DNA and requires template and primer. Additionally, the enzyme lacks 3’→5’ exonuclease activity.
Purified from a strain of E. coli that carries a T4 DNA Polymerase, Exonuclease Minus expression plasmid.
Heat inactivation: 70°C for 15 minutes.
Enzyme and buffer should be stored at -20°C. Enzyme is stable for 12 months if handled properly.