The TargetAmp™ 1-Round Biotin-aRNA Amplification Kit 105* produces microgram amounts of biotin-antisense RNA (biotin-aRNA, also called biotin-cRNA) from as little as 25 ng of total cellular RNA. The kit provides all reagents for the preparation of biotin-aRNA (except reverse transcriptase and RNA purification columns) and uses an improved Eberwine-type linear amplification method1 to achieve at least 5,000-fold amplification of the poly(A) RNA present in a total cellular RNA sample. The entire procedure can be completed in 1 day.
Table 1. A TargetAmp™ Kit 105 reaction produces high yields of biotin-aRNA from as little as 25 ng of input total RNA. Results represent the average of multiple experiments.
|Amount of Input HeLa Total RNA||Biotin-aRNA Yield from HeLa Total RNAa|
|25 ng||4.1 µg|
|100 ng||13.7 µg|
|500 ng||64.9 µg|
a. Approximately 2% of HeLa total RNA is poly(A) RNA.
Figure 1. Correlation of expression ratios obtained from qPCR and from microarrays hybridized to aRNA prepared with the TargetAmp™ 105 kit. X axis, log2 expression ratios determined from Human Genome U133 Plus 2.0 Arrays (Affymetrix) hybridized to targets prepared by the TargetAmp 105 kit; Y axis, log2 expression ratios determined by the MAQC, using TaqMan® assays. The high correlation obtained (r = 0.936) indicates the excellent fidelity of the amplification process.
*TargetAmp™ aRNA Amplification Products are covered by intellectual property licensed to Epicentre Technologies Corporation from Johnson & Johnson Pharmaceutical Research & Development, L.L.C., and by intellectual property sublicensed to Epicentre Technologies Corporation from Incyte Corporation.
For research use only. Some applications in which this product may be used may be covered by patents or patent applications applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a license, depending upon the particular application and country in which the product is used.