Library Pooling & Superpooling

• Speed discovery and reduce screening costs.
• Use PCR to screen pools of clones to identify clone(s) of interest.

Automated Colony Picking, Re-arraying, & Duplicating

• Consistent assessment of colony roundedness, size and color.
• Convenient arrangement in 384-well plates.

Gridding & High Density Colony Filters

• DNA from cells robotically printed and fixed onto nylon filters.
• Rapid screen for clone of interest by hybridization.

BAC Library Miniprep DNA

Ultra pure High Molecular Weight Genomic DNA

• Easily screen the clones individually.
• Help tools for gene discovery and BAC end sequencing.

Custom Sequencing

• High throughput sequencing on ABI 3730x sequencers.
• Complete library analysis more quickly.

Random Shear BAC Libraries

• Superior replacement for partial digestion methods.
• Unbiased and gap-free.
• Complete coverage with a single library.
• Large inserts: >100kb.
• Truly representative of input DNA.
• Faster analysis; dramatically reduced analysis costs.

Partial Digestion BAC Libraries

• High-quality, large-insert partial digestion BAC library (conventional BAC library).
• Guaranteed average insert size will be between 120 Kb~ 200+ Kb.
• Fast turn-around time.

Metagenomic BAC and Fosmid Libraries

Lucigen has successfully developed proprietary techniques for preparing High Molecular Weight (HMW) genomic DNA from many sources including collected microbe samples from plant and animal hosts; soil samples; marine species: plankton, algae, sponges; and other environmental samples; and extremophiles.

Fosmid Libraries

• Random sheared insert DNA eliminates bias.
• Insert size up to 90kb.

Large Insert Libraries

• Libraries created with stable inserts even from AT-rich genomes.
• Inserts up to 30kb in the pJAZZ^® linear vector.

Shotgun Libraries

• Libraries constructed with as little as 1 ng of target DNA.
• Inserts stabilized in pSMART^® transcription-free vectors.