Request Free No Obligation Quote Custom Sequencing & Library Construction

Lucigen is recognized worldwide as the only source of unbiased, high fidelity custom shotgun, BAC, and fosmid libraries, constructed using our proprietary cloning technologies and exclusive transcription-free vectors. We now include high throughput (96-well) library sequencing, for a one-stop, economical, fast-turnaround custom service. Library sequencing is done using ABI 3730xl sequencers.

  • Perfect for shotgun BAC, metagenomic, or small genomic libraries.
    Simply provide us with your BAC, fosmid, genomic, or phage DNA. We construct the library, sequence it, and provide you with the sequence and the clones.

  • Dramatically reduced finishing costs.
    Transcription-free, high stability vectors (Figure 1) with Lucigen’s patented CloneSmart® technology prevent lost genes and sequence deletions or rearrangements, even with AT-rich inserts, toxic genes, nucleotide repeats, and other difficult-to-clone DNAs.

  • Unequalled expertise in library construction and sequencing.
    Technologies pioneered by Lucigen – including Random Shear BAC library construction, NanoClone® library construction from trace amounts of DNA, and the linear pJAZZ® vector – have greatly improved the quality and usefulness of genomic libraries. We have constructed hundreds of shotgun, fosmid, and BAC libraries for satisfied customers worldwide. Our high throughput sequencing group has many years of automated sequencing experience, including whole genome shotgun libraries with large and small inserts and transposon sequencing. The head of our sequencing group previously managed the University of Wisconsin-Madison, Genetics Sequencing and Genomics Services Center (GSGSC).

  • Confidentiality assured.
    Lucigen provides a confidentiality agreement to guarantee the complete security of all client information, project data, and biological samples.
Panel A  
pSMART GC HK vector  
   
Panel B  
pJAZZ ChimeraFree linear vectors
Figure 1. Lucigen’s high stability, chimera-free cloning vectors for custom library construction & sequencing.

Panel A. pSMART® ChimeraFree vectors (clone up to 8-10 kb). The pSMART vectors are available in either high copy or low copy versions. G-tailed insert DNA is ligated to the C-tailed vector termini, ensuring only one insert per vector. Ori, origin of replication; Kan, Kanamycin resistance gene. Approximate positions of sequencing primers and CloneSmart transcription terminators (T) are indicated. The low copy vector includes ROP, Repressor of primer (lowers plasmid copy number).

Panel B. pJAZZ ChimeraFree linear vectors (clone up to 30 kb). The copy number is ~4-10/cell prior to induction; it is increased by approximately 10-20 fold by a simple chemical induction step in the included BigEasy®-TSA™ Cells. (1) Before processing, the pJAZZ vector (12.9 kb) contains a lacZ stuffer fragment. (2) After processing, the vector has 3’-C tails and dephosphorylated ends. G-tailed insert DNA is ligated to the C-tailed vector arms, ensuring only one insert per vector. The left arm is 10 kb and the right arm is 2.2 kb. TelN, protelomerase gene; repA, replication factor and origin of replication; cB, regulator of copy number; Camr, chloramphenicol resistance gene; Kanr, kanamycin resistance gene. Approximate positions of CloneSmart transcription terminators (T) are indicated.

Contact Lucigen for a free quote on Custom Library Construction & Sequencing