Custom Services from Lucigen
Timely • Flexible • Confidential

Don’t have the time, staff, or expertise to do it yourself?
Let us do it for you. Contact us for a free quote on any of our custom services.

Custom Competent Cells

Bulk discounts, Any E. coli cells you need

  • Electrocompetent cells with up to 4 x 1010 cfu/µg transformation efficiency.
  • Dispensed at any volume in tubes, plates, or strips.
  • Fast turn-around time.
  • Cells available for many applications:
  • protein expression
  • cloning library construction
  • phage display

Custom Libraries

Random Shear BAC Libraries

  • Superior replacement for partial digestion methods.
  • Unbiased and gap-free.
  • Complete coverage with a single library.
  • Large inserts: >100kb.
  • Truly representative of input DNA.
  • Faster analysis; dramatically reduced analysis costs.

Large Insert Libraries

  • Libraries created with stable inserts even from AT-rich genomes.
  • Inserts up to 30kb in the pJAZZ® linear vector.

Fosmid Libraries

  • Random sheared insert DNA eliminates bias.
  • Insert size up to 90 kb.

Shotgun Libraries

  • Libraries constructed with as little as 1 ng of target DNA.
  • Inserts stabilized in pSMART® transcription-free vectors.

 

 

SUPPORTING Services

Library Pooling & Superpooling

  • Speed discovery and reduce screening costs.
  • Use PCR to screen pools of clones to identify clone(s) of interest.

Automated Colony Picking, Re-arraying, & Duplicating

  • Consistent assessment of colony roundedness, size and color.
  • Convenient arrangement in 384-well plates.

Gridding & High Density Colony Filters

  • DNA from cells robotically printed and fixed onto nylon filters.
  • Rapid screen for clone of interest by hybridization.

Custom Sequencing

  • High throughput sequencing on ABI 3730x sequencers.
  • Complete library analysis more quickly.

 

Contact Lucigen for a free quote on any of our custom services.

Lucigen provides a confidentiality agreement to guarantee the complete security of all client information, project data, and biological samples.