Gridding & High Density Colony Filters

To facilitate screening large numbers of DNA clones, Lucigen can also robotically print viable, bacterial cells from 96 or 384 well plates onto high-density, 22.2 × 22.2 cm nylon filters. After overnight growth of the clones, the filters are processed. As a result, DNA from each clone is permanently fixed to a known position on the filter, allowing rapid screening using standard DNA hybridization procedures. Positive clones can then be identified and retrieved from the original plates. Each nylon filter can be screened 3-5 times. Lucigen provides 22.2 cm × 22.2 cm or 8 × 12 cm filters printed at a choice of two densities. All clones are printed in duplicate. We can also provide customized filters to meet your needs. See Figure 3 for a representative example of a Lucigen gridded filter.

Table 1. Types of High Density Colony Filters

*We can meet customer’s special needs: for example, different spotting patterns, single spotting, etc.

Figure 3. Example of a Lucigen gridded filter (4x4). The membrane (filter) is divided into 6 fields. The spacing of colonies is slightly wider between the fields. Each field contains 384 squares. The 384 squares represent the row and column identification of the BAC clone. Within each square there are 16 positions where 8 clones are spotted in duplicate. The pattern of the spotted clones will generate the plate address of the BAC. The numbers in the spotting pattern indicate the plate numbers. Each grid corresponds to each well of the original 384-well plates, starting from A1 from the left-top corner. Each Lucigen filter includes the identification code of every clone within the grid.

Contact Lucigen for a free quote on any of our custom services.