As the leader in advanced products for molecular biology, Lucigen offers the best performance, price, and value in competent cells for cloning and expression…from the unequalled high performance of E. cloni® 10G SUPREME Electrocompetent Cells for ultra high efficiency cloning, to the only choice for successful expression of toxic proteins: the new OverExpress™ Competent Cells. No other supplier offers the spectrum of competent cells for your cloning and expression needs.
Table 1: Competent Cell strains, efficiencies, and applications
| Application |
Strain
|
Chem. Comp. Efficiency
|
Electrocomp. Efficiency
|
| Cloning |
|
|
|
| The most demanding cloning projects |
E. cloni 10G SUPREME Electrocompetent
|
|
>4 X 1010
|
| Libraries, difficult-to-clone DNA |
E. cloni 10G ELITE Electrocompetent
|
|
>2 X 1010
|
| General, high efficiency cloning |
E. cloni 10G CLASSIC Electrocompetent
|
|
>5 X 109
|
| Routine cloning and subcloning |
E. cloni 10G Chemically Competent
|
>1 X 109
|
|
|
E. cloni 5-alpha Chemically Competent
|
>1 X 108
|
|
| Simple subcloning, plasmid propagation |
E. cloni 10G ELITE 10G Chemically Competent, Subcloning Grade |
>1 X 106
|
|
| ssDNA production, LacIq repression |
E. cloni 10GF'
|
>5 X 108
|
>2 X 1010
|
| Linear vector cloning |
BigEasy-TSA Electrocompetent
|
|
>4 X 1010
|
| BAC cloning |
BAC-Optimized Replicator v2.0 Electrocompetent
|
|
>2 X 1010
|
| Expression |
|
|
|
| Phage display, expression and cloning |
TG1 Electrocompetent,
SS320 (MC1061 F') Electrocompetent,
ER2738 Electrocompetent
|
|
>2 X 1010
|
| Routine expression (T7 promoter) |
E. cloni EXPRESS BL21(DE3)
|
> 1 x 107
|
>5 X 109
|
| Routine expression (Tac promoter) |
E. cloni 10GF'
|
>5 X 108
|
>2 X 1010
|
| Expression of toxic and membrane proteins |
OverExpress C41/C43 (DE3)
|
> 1 x 106
|
>1 X 1010
|
| Expression of very toxic proteins |
OverExpress C41/C43 (DE3)pLysS
|
> 1 x 106
|
>1 X 109
|
Click on the categories below to see why researchers worldwide are using Lucigen competent cells.
Competent Cells for Cloning
- NEW! SS320 (MC1061 F') Electrocompetent Cells: >4 X 1010 cfu/µg DNA
- NEW! ER2738 Electrocompetent Cells: >2 X 1010 cfu/µg DNA
- E. cloni 96-well 10G Chemically Competent Cells: >1 X 108 cfu/µg DNA
- E. cloni 5-alpha Chemically Competent Cells: > 1 x 108 cfu/µg
- TG1 Electrocompetent Cells: > 2 x 1010 cfu/µg
- BAC-Optimized Replicator™ v2.0 Electrocompetent Cells: > 2 x 1010 cfu/µg DNA
- E. cloni 10G BAC-Optimized Electrocompetent: >1 x 1010 cfu/µg 150 kb BAC DNA
- E. cloni FLASH 10G Electrocompetent in Cuvettes: > 2 x 1010 cfu/µg DNA
- E. cloni 10G CLASSIC Electrocompetent: > 5 x 109 cfu/µg DNA
- E. cloni 10G SUPREME Electrocompetent: > 4 x 1010 cfu/µg DNA
- E. cloni 10G or 10GF' ELITE Electrocompetent: > 2 x 1010 cfu/µg DNA
- BigEasy-TSA Electrocompetent Cells > 4 x 1010 cfu/µg DNA
- E. cloni 10GF' Chemically Competent: > 5 x 108 cfu/µg DNA
- NEW! E. cloni 10G Chemically Competent: > 1 x 109 cfu/µg DNA
- NEW! E. cloni 10G Chemically Competent, Subcloning Grade: > 1 x 106 cfu/µg DNA
E. cloni EXPRESS Competent Cells for Routine Protein Expression
- E. cloni EXPRESS BL21(DE3) Electrocompetent: > 5 x 109 cfu/µg DNA
- E. cloni EXPRESS BL21(DE3) Chemically Competent: > 1 x 107 cfu/µg DNA
OverExpress Competent Cells for Toxic Protein Expression
- OverExpress C41 or C43 Electrocompetent: > 1 x 1010 cfu/µg DNA
- OverExpress C41 or C43 Chemically Competent: > 1 x 106 cfu/µg DNA
High Yield Ligation & Transformation Systems
- UltraClone™ DNA Ligation & Transformation Kit with E. cloni 10G Electrocompetent or Chemically Competent Cells.
Custom Competent Cell Service
Table 2: Competent Cell Characteristics
|
Cloning Strains
|
| Strain Attribute |
10G (DH10B)
|
10GF' (DH10BF')
|
BAC-Opt v2 Replicator
|
5-alpha (DH5alpha)
|
BigEasy-TSA
|
| T1 Phage Resistant |
|
|
|
|
|
| Blue/White Screen |
|
|
|
|
|
| LacIq Repressor |
|
|
|
|
|
| Endonuclease I Deficient |
|
|
|
|
|
| Methylated DNA Cloning |
|
|
|
|
|
| Express M13 Phage (F') |
|
|
|
|
|
| RecA Deficient |
|
|
|
|
|
|
|
| Strain Attribute |
TG1
|
S320 (MC1061 F')
|
ER2738 |
EXPRESS BL21 (DE3)
|
BL21 (DE3) pLysS
|
BL21 (DE3) pLysE
|
C41/C43 (DE3)
|
C41/C43 (DE3) pLysS
|
| Blue/White
Screen |
|
|
|
|
|
|
|
|
| LacIq Repressor |
|
|
|
|
|
|
|
|
| Protease Deficient |
|
|
|
|
|
|
|
|
| Methylated DNA Cloning |
|
|
|
|
|
|
|
|
| Express M13 Phage (F') |
|
|
|
|
|
|
|
|
