Metagenomic BAC and Fosmid LibrariesRequest Free No Obligation Quote

  • High molecular weight DNA prepared from exotic sources

  • Large insert libraries produced from minimal material

Lucigen has successfully developed proprietary techniques for preparing High Molecular Weight (HMW) genomic DNA from many sources including collected microbe samples from plant and animal hosts; soil samples; marine species: plankton, algae, sponges; and other environmental samples; and extremophiles.

The high quality HMW genomic DNA can be used to manufacture Random Shear BAC and fosmid libraries (Fig. 1).

High molecular weight genomic DNA isolated into pSMART BAC vector.
Figure 1. High Molecular Weight genomic DNA was isolated from micro-algae, randomly sheared, size-selected 100~300 kb, and cloned into the pSMART BAC vector. DNA from minipreps was digested with NotI to excise inserts. The vector band is visible at 7 kb.

All BAC/fosmid libraries are delivered as frozen clones in 384- or 96-well plates with barcode labels on the lids and sides and shipped in Lucigen’s library storage boxes on dry ice. A library construction report, technical support, and the directions for safe handling of the custom BAC library are also provided.


Contact Lucigen for a free quote on any of our custom services.