Automated Colony Picking, Re-arraying, & Duplicating

Figure 2. A robot picking pin picks a BAC clone from a Q-tray plate of Genetix robot.

Lucigen offers custom colony picking, re-arraying and duplicating, either as a stand-alone service or as part of our library construction services (Random Shear or conventional BAC, fosmid, GapFree™ shotgun, and NanoClone® libraries). Colony picking is an automated method for transferring colonies from a solid to liquid medium in 96- or 384-well plates. From directly transformed clones or frozen glycerol stocks, colonies are first grown on an agar tray and then robotically picked into plates for further growth and storage. Lucigen’s Genetix picking robots uses a camera to assess colony roundedness, size and color before picking and transferring to the 384-well plate. BAC libraries are delivered as clones frozen in 384-well plates. The average BAC insert size is >100 kb for random-sheared DNA or >150 kb for conventional BAC clones. Our well-trained engineers and technicians achieve the highest robot picking accuracy and guarantee a library array without empty wells.

Contact Lucigen for a free quote on any of our custom services.