MasterAmp™ Taq DNA Polymerase is a thermostable DNA polymerase derived from Thermus aquaticus. Having optimal activity at temperatures above 70°C, it was the first thermostable enzyme used for PCR. It has an intrinsic 5´ to ;3´ structure-dependent exonuclease activity, but lacks 3´ to ;5´ proofreading exonuclease activity. The enzyme is highly purified using a proprietary procedure that virtually eliminates contaminating bacterial DNA.
Unit Definition: One unit of MasterAmp Tth DNA Polymerase converts 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 70°C under standard assay conditions.
Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 0.5% Tween 20, 0.5% NP-40, and 1 mM DTT.
Quality Control: MasterAmp Tth DNA Polymerase is tested for the absence of detectable nonspecific exo- and endonuclease and RNase activities, and are functionally tested in PCR.
*Purchase of MasterAmp™ Taq DNA Polymerase includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights (such as 5´ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. Covered by issued and pending patents. For complete license statement, see p. iv.
**Covered by issued and/or pending patents.