Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5´ to ;3´ direction. Its activity is dependent on Mg2+. Rec J Exonuclease can be heat-inactivated by incubation at 65°C for 20 minutes.
ApplicationsRemoval of primers from completed PCR. Degradation of single-stranded linear DNA in dsDNA and plasmid preps.
Unit Definition: One unit of Rec J Exonuclease catalyzes the release of 1 nmol of acid-soluble nucleotides from activated single-stranded calf thymus DNA in 30 minutes at 37°C under standard assay conditions.
Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 0.1 M NaCl, and 0.1% Triton® X-100.
10X Reaction Buffer: 330 mM Tris-acetate (pH 7.5), 660 mM potassium acetate, 100 mM magnesium, acetate, and 5.0 mM DTT.
Quality Control: Rec J Exonuclease is free of detectable RNase and contaminating DNA endonuclease activities.