The T7 RNA Polymerase produces defined RNA by in vitro transcription of dsDNA that is downstream of the specific T7 RNA polymerase promoter. This enzyme preparation is highly purified, and exhibits excellent promoter specificity.
Unit Definition: One unit of RNA polymerase catalyzes the incorporation of 1 nmol of ribonucleoside triphosphate into RNA in 1 hour at 37°C under standard assay conditions using a DNA template with the appropriate T7 promoter.
Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1.0 mM DTT, 0.1 mM EDTA, and 0.1% Triton® X-100.
Quality Control: The T7 RNA polymerase is tested in in vitro transcription reactions, and is free of detectable exo- and endonuclease and RNase activities, and E. coli RNA polymerase activity.