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Sygnis TruePrime™ Single Cell WGA Kit version 2.0

Single cell whole genome amplification (WGA) with less bias making this kit ideally suited for next generation sequencing applications.

  • High sensitivity: Typical yield is greater than 5 µg when starting with a single mammalian cell.
  • Reduced amplification bias and no primer artefacts: Primer-free amplification method utilizes a combination of TthPrimPol Primase (to synthesize primers) and Phi29 DNA polymerase.
  • Well suited for next generation sequencing: Tested in Illumina and Ion Torrent NGS workflows.
  • Easy and reliable
  • Insensitive to external DNA contamination
  • Ordering

  • Specifications

  • Manuals

  • Resources

Product Description Size Cat. No. Price Quantity BUY
Sygnis TruePrime™ Single Cell WGA Kit version 2.0  25 rxn SYG351025 $560.00
   100 rxn SYG351100 $1890.00
ORDER INFORMATION
Each TruePrime™ Single Cell WGA Kit version 2.0 contains: Buffer L1, DTT, Buffer N, Reaction Buffer, dNTPs, Water, Enzyme 1, Enzyme 2, Control 1, and Control 2. The complete manual is available under the Manual tab. Lucigen is an authorized distributor of Sygnis products in the US.

TruePrime™ Single Cell WGA version 2.0 kit is intended for molecular biology use only and in vitro use only. This product is not intended for diagnosis, prevention or treatment of a disease in human beings or animals.

Technical Specifications:
Typical DNA yields from a TruePrime™ Single Cell WGA kit version 2.0 reaction are about 5 µg per 50 µl reaction when starting from one or a few cells. Yields and kinetics will vary if crude or un-quantified samples are amplified. Reactions without input DNA (no template controls) do not produce any amplification product during a 3 hour reaction. Mean product length is greater than 10 kb.

Quality Control:
Each batch of a TruePrime™ Single Cell WGA Kit version 2.0 is tested against predetermined specifications to ensure consistent product quality. Enzymes used in the kit have been tested separately to ensure adherence to specifications.

Shipping and Storage:
Kits are shipped on dry ice. Upon receipt, the kit should be stored immediately at -20°C in a non-frost-free (constant-temperature) freezer. When stored under these conditions and handled correctly, the products can be kept at least six months after shipping without showing any reduction in performance. For longer periods of time, store the kit at -80°C.

Document File Name Type
Manual Sygnis-TruePrime-Single-Cell-WGA-Handbook PDF

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