“Taq98 is my go-to polymerase for routine PCR”
-Christopher Jurgenson, Ph.D., Post-Doctoral Associate
Molecular, Cellular and Developmental Biology, Yale University

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Taq98™ Hot Start 2X Master Mix

  • Unmatched ability to amplify GC-rich and other tough templates
  • Thermostability at  98°C ensures denaturation of high GC content targets
  • Hot Start Polymerase format eliminates primer dimer formation
  • Convenient 2X Master Mix
  • Great value and convenient pack sizes

Ordering

Product Description Size Cat. No. List Price Quantity BUY
Taq98™ Hot Start 2X Master Mix  50 rxn 30057-0 $0.00
   250 rxn 30057-1 $173.00
   500 rxn 30057-2 $312.00
ORDER INFORMATION
Taq98™ Hot Start is provided in a 2X Master Mix format that withstands 98°C denaturation. All required buffer components, enzyme, Mg++ and dNTP’s are all present in optimized concentrations. Only template DNA, primers and nuclease-free water are required to set up the reaction. Please see the user manual for composition information. Standard reaction size is 50 µL.

Description

Amplify your toughest DNA templates with Taq98™ Hot Start 2X Master Mix – minimize non-specific amplification.

Extreme thermostability. Cycle at 98°C to denature the toughest templates. The added PCR enhancer improves amplification and thermostability to give you the best PCR product possible.

Hot Start function eliminates Primer Dimer formation! Robust hot-start technology virtually eliminates unwanted products such as primer dimers that can result from non-specific amplification. Worry free set up of Taq98 reactions at room temp.

Tough PCR made simple: Templates with tough structure, such as promoter regions, can be hard to reliably amplify (Figure 1, C. fimi gDNA). GC-rich templates can be just as difficult (figure 2). Taq98 is the best option for taking control of your amplification.

Easy to use. Taq98™ comes with everything you need all in one vial. Just add your primers and template!

Taq98 Hot Start PCR Tough Templates: Human

Figure 1. Taq98™ Hot Start is compared with other common hot start products in amplifying eight difficult templates from human genomic DNA.

Hot Start PCr: Taq98 Tough Templates, c. fimi

Figure 2.Taq98 shows superior performance in amplifying a selection of genes from Cellumonas Fimi genomic DNA (~75% GC). Other enzymes show only extremely weak amplification or primarily primer dimers, in contrast with Taq98 which demonstrates robust amplification and minimal spurious banding.

Resources

Document File Name Type
Manual MA126-Taq98-Hot-Start PDF
Poster Taq98-Hot-Start-poster-2011 PDF
Product Flyer Taq98-2011 PDF
Citations Taq98™ Hot Start 2X Master Mix - Citations LINK
PDF MSDS PDF

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