Leaders in Random Shear BAC Library Construction
Your project can benefit from our expertise in library construction, screening, cloning and other genomic analysis. Lucigen scientists have constructed over 1,000 libraries including more than 100 Random Shear BAC Libraries from metagenomes, microbes, and plant and animal species for clients all over the world.
Lucigen can also screen your library or provide the tools for you to efficiently screen libraries on your own. Complete package service, such as BAC library construction, library screening, genome-wide BAC end sequencing, building the BAC minimum tile of a genome or chromosome, position-cloning of candidate genes/QTLs/transgenic loci, or complete NextGen Roche 454 or Sanger sequencing and analysis are available. We can also create custom clones from otherwise uncloneable DNA.
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Contact us to find out how we can help your research.
BAC/Fosmid Libraries |
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Unbiased and Gap-Free. Complete Coverage with a Single Library Large inserts: >100kb. |
Random sheared insert DNA eliminates bias. Insert size up to 90 kb. |
High-quality, large-insert, partial digestion BAC Libraries Guaranteed average insert size: 120 kb~200+kb. Fast turn-around time. |
NextGen & Sanger Sequencing |
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Roche 454 multiplexed data As little as 1 μg required Cost effective results |
Entire BAC/fosmid sequenced efficiently. Chimera-free clones provided. |
Up to 700 bp per run. Minimum of 90% successful reads. Any scale: single read to genome-wide BAC end sequencing. |
High throughput sequencing on ABI 3730x sequencers. |
Other Libraries/Cloning |
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Libraries created with stable inserts even from A/T-rich genomes. Up to 30 kb in linear pJAZZ® Vector. |
Large/Difficult Insert Cloning Repetitive, G/C- or A/T-rich inserts cloned efficiently. Up to 30 kb in linear pJAZZ® Vector. |
Libraries constructed with as little as 1 ng of target DNA. Inserts stabilized in pSMART® transcription-free vectors. |
DNA isolated from environmental or complex samples. BAC or fosmid libraries created from minimal starting material. |
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Library Screening |
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Speed discovery and reduce screening costs. Convenient. No radioactive materials needed. Use PCR to screen pools of clones to identify clone(s) of interest. |
DNA from cells robotically printed and fixed onto nylon filters. Rapid screen for clone(s) of interest by hybridization. |
Picking, re-arraying, duplicating. Consistent assessment of colony roundedness, size and color. Convenient arrangement in 384-well plates. |
Lucigen’s experts screen your library for your sequence of interest. Confidential, cost- and time-efficient. |
DNA Prep |
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Ultra pure HMW Genomic DNA From either 96-well or 384-well plates. DNA for gene discovery and BAC end sequencing. |
Genomic DNA up to Megabase-size. Prepared from any source. For Next-Gen Sequencing, transgene construction, etc. Large-scale prep for individual circular or linear BACs. |
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