Protein Expression

Simplify your cloning and protein expression work with Lucigen’s complete cloning and expression systems. We also offer highest efficiency competent cells for routine cloning and expression as well as competent cells that can express difficult or toxic proteins.

Expressioneering™ Technology is an entirely new way to radically simplify cloning and expressing recombinant proteins. Expressioneering Technology uses in vivo homologous recombination to seamlessly clone PCR amplified DNA into Expresso® vectors without the need for enzymes or purification steps. Expresso Cloning and Expression Kits are supplied complete with pre-processed vectors and high-efficiency competent cells to save you time and money.

Also see Competent Cells for Phage Display.

Expressioneering™ Technology

Expresso® CMV Cloning and Expression System » NEW The Expresso® CMV Cloning and Expression System combines instant, enzyme-free, directional cloning with a “no scar” vector for eukaryotic gene expression using Expressioneering™ Technology.

Expresso® Rhamnose Cloning & Protein Expression System » NEW Directional PCR cloning in seconds with Expressioneering Technology. pRham™ vectors with tunable rhaPBAD promoter provide tightly controlled expression of N- or C-terminal 6xHis tagged proteins. One competent cell strain is provided for cloning and expression, enabling high throughput protein expression. Enhanced expression and solubility of difficult target proteins with optional cleavable SUMO fusion tag.

Expresso® SUMO Cloning and Expression Systems » Enhanced expression of difficult target proteins and improved solubility of inclusion body prone proteins with cleavable 6xHis-SUMO fusion tag. Efficient SUMO tag cleavage with SUMO Express Protease to generate native protein. Easily remove cleaved SUMO tag and Protease by metal affinity chromatography. Choice of vectors under tight control of inducible T7 or tunable Rhamnose promoters.

Expresso® T7 Cloning and Expression System » Enzyme-free directional PCR cloning in seconds. Complete system includes cloning-ready vectors and competent cell lines. Tightly controlled expression of N- or C-terminal 6xHis tagged proteins. Quickly purify expressed proteins by metal affinity chromatography.

Competent Cells for Protein Expression

OverExpress™ C41(DE3) and C43(DE3) Competent Cells » Express difficult or even toxic proteins using any E. coli T7 vector. Available as chemically competent or electrocompetent cells.

E. cloni® EXPRESS BL21(DE3) Competent Cells » For routine protein expression using any E. coli T7 expression vector. Available as chemically competent or highest efficiency electrocompetent cells.

HI-Control™ Competent Cells » Greater control of protein expression using the LacI repressor in two popular cell lines.