Partial Digestion BAC Libraries

• High-quality, conventional BAC library
• Economical alternative to Random Shear method

• BAC/Fosmid Libraries
  • Random Shear BAC
  • Novel (large insert) Fosmid
  • Partial Digestion BAC
• Sequencing
  • NextGen Sequencing
  • BAC end Sequencing
  • BAC Shotgun Sequencing
  • Sanger DNA Sequencing
• Other Libraries/Cloning
  • Difficult Genome
  • Large/Difficult Insert Cloning
  • NanoClone ™ & GapFree Shotgun
  • Metagenomic
• Library Screening & Tools
  • Pooling & Superpooling
  • Gridding & Colony Filters
  • Colony Preparation
  • Library Screening
• DNA Prep
  • BAC Miniprep / DNA Prep
  • HMW Genomic DNA Prep

Figure 1 shows a partial digestion mouse genomic BAC library constructed at Lucigen.

A typical 5~10X BAC partial digestion library is constructed using one or more of the following restriction enzymes: HindIII, EcoRI, BamHI. Additional coverage and/or additional enzyme digestion is available.

All libraries are delivered as frozen BAC clones in 384- or 96-well plates with barcode labels on the lids and sides and shipped in Lucigen's library storage boxes on dry ice. A library construction report, technical support, and the directions for safe handling of the custom BAC library are also provided.

Figure 1. High Molecular Weight genomic DNA was isolated from mouse blood, partial digested with HindIII, size-selected 100~400 kb, and cloned into the pSMART BAC vector. DNA from minipreps was digested with NotI to excise inserts. The vector band is visible at 7 kb.

Contact Lucigen for a free quote on any of our custom services.