- Lucigen’s scientists will create an efficient discovery tool for your research.
- Find your clone in as few at 59 PCR reactions.
Lucigen’s BAC library pooling and superpooling system (Figure 1) enables quick and efficient identification of a sequence of interest from even large libraries. The system consists of pooled clone DNA (from a BAC, fosmid, or other library) arranged in 96-well plates and organized so the specific library address of a sequence of interest can be determined with as few as 59 PCR amplification reaction. Most clones can be identified with only two rounds of PCR screening. We can also use pooling patterns designed by a customer.
PROCEDURE OVERVIEW
Round 1 Run PCR tests on the SuperPool Collection Tubes/Plates to determine which SuperPool contains your clone-of-interest.
In this example, SP1 contains the clone-of-interest.
Round 2 Run PCR tests on the appropriate Plate-Row-Column Pools Collection Plate to determine the location of your clone-of-interest. In this example, P2, RC, and C9 in Plate-Row-Column Pools Collection Plate from SuperPool 1.
Figure 1. Overview of screening Lucigen's Pools and Superpools.
DONE! Analyze BAC Clone from well at the coordinates from Round 2.
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