Custom Sequencing & Library Construction

NEW!

• BAC/Fosmid Libraries
  • Random Shear BAC
  • Novel (large insert) Fosmid
  • Partial Digestion BAC
• Sequencing
  • NextGen Sequencing
  • BAC end Sequencing
  • BAC Shotgun Sequencing
  • Sanger DNA Sequencing
• Other Libraries/Cloning
  • Difficult Genome
  • Large/Difficult Insert Cloning
  • NanoClone ™ & GapFree Shotgun
  • Metagenomic
• Library Screening & Tools
  • Pooling & Superpooling
  • Gridding & Colony Filters
  • Colony Preparation
  • Library Screening
• DNA Prep
  • BAC Miniprep / DNA Prep
  • HMW Genomic DNA Prep

Lucigen is recognized worldwide as the only source of unbiased, high fidelity custom shotgun, BAC, and fosmid libraries, constructed using our proprietary cloning technologies and exclusive transcription-free vectors. We now include high throughput (96-well) library sequencing, for a one-stop, economical, fast-turnaround custom service. Library sequencing is done using ABI 3730xl sequencers.

• Perfect for shotgun BAC, metagenomic, or small genomic libraries.
  Simply provide us with your BAC, fosmid, genomic, or phage DNA. We construct the library, sequence it, and provide you with the sequence and the clones.
• Dramatically reduced finishing costs.
  Transcription-free, high stability vectors (Figure 1) with Lucigen’s patented CloneSmart^® technology prevent lost genes and sequence deletions or rearrangements, even with AT-rich inserts, toxic genes, nucleotide repeats, and other difficult-to-clone DNAs.
• Unequalled expertise in library construction and sequencing.
  Technologies pioneered by Lucigen – including Random Shear BAC library construction, NanoClone^® library construction from trace amounts of DNA, and the linear pJAZZ^® vector – have greatly improved the quality and usefulness of genomic libraries. We have constructed hundreds of shotgun, fosmid, and BAC libraries for satisfied customers worldwide. Our high throughput sequencing group has many years of automated sequencing experience, including whole genome shotgun libraries with large and small inserts and transposon sequencing. The head of our sequencing group previously managed the University of Wisconsin-Madison, Genetics Sequencing and Genomics Services Center (GSGSC).
• Confidentiality assured.
  Lucigen provides a confidentiality agreement to guarantee the complete security of all client information, project data, and biological samples.

Figure 1. Lucigen’s high stability, chimera-free cloning vectors for custom library construction & sequencing.
Panel A	Panel B
Panel A. pSMART® ChimeraFree vectors (clone up to 8-10 kb).	Panel B. pJAZZ ChimeraFree linear vectors (clone up to 30 kb).

Contact Lucigen for a free quote on Custom Library Construction & Sequencing