Complete your large-insert BAC cloning and library construction projects with unprecedented accuracy, reliability and efficiency using CopyRight® v2.0 BAC cloning kits with pSMART® BAC vector and BAC-Optimized Replicator™ v2.0 Electrocompetent Cells.
CopyRight kits are specifically designed for optimal cloning efficiency and versatility. Minimize cloning bias and clone otherwise toxic genes with the pSMART BAC vector (Figure 1), designed with CloneSmart® transcription-free technology to stabilize difficult inserts. The lacZ-sacB stuffer fragment eliminates uncut vector from recombinants resulting in zero background, and inducible copy number amplification (20-50X) allows high DNA yields.
Clone a single target, or generate a complete library (Figure 2) with confidence. The CloneSmart design maintains "unclonable" inserts, ensuring sequences are not lost or rearranged in the cloning process.
Figure 1. CopyRight vector pSMART BAC. ori2, repE, IncC - single-copy origin of replication; oriV - inducible origin of replication; par A,B,C - partition genes; Cm^r - chloramphenicol-resistance gene; cosN - lambda packaging signal; T - CloneSmart transcription terminators; sacB -sucrase gene. Approximate positions of sequencing primers and transcription terminators are indicated.
Figure 2. Arabidopsis Random Shear BAC Library in the pSMART BAC vector. 7,680 clones were obtained with an average insert size of 105 kb and ~5X genome coverage.
Convenience and Performance
Eliminate tedious vector and competent cell preparation, and time-consuming QC experiments. The easy-to-use CopyRight v2.0 kits contain optimized reagents, ligation-ready vector, prepared electrocompetent cells and detailed instructions for high-efficiency cloning.
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