Figure 1. EconoTaq PLUS GREEN Master Mix vs. GoTaq® Green master mix (Promega) in PCR. PCR was performed according to the manufacturer’s recommendations using primers specific for the indicated genes. Panel A, 0.7 kb prokaryotic single-stranded DNA binding protein (genomic DNA); Panel B, 2.7 kb prokaryotic DNA polymerase A (genomic DNA); Panel C, 4.5 kb gene (plasmid DNA).
Figure 2. GC-rich regions from human genomic DNA were PCR amplified using 98° C denaturation.
Figure 3. Easy visualization with EconoTaq PLUS GREEN. During electrophoresis, the green loading color separates into a slow-moving blue dye and a fast-moving yellow dye.
Some applications in which Lucigen's EconoTaq PLUS GREEN and EconoTaq PLUS 2X Master Mixes can be used may be covered by patents issued and applicable in the United States and certain other countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used. The PCR process is the subject of European Patent Nos. 201,184 and 200,262 owned by Hoffman-LaRoche. Those patents expired on March 28, 2006. The corresponding PCR process patents in the United States expired on March 29, 2005. It is the sole responsibility of the buyer to ensure that use of the product does not infringe the patent rights of third parties.
PCR Activity: EconoTaq PLUS GREEN & EconoTaq PLUS 2X Master Mixes are tested in DNA amplification using a variety of templates and primers.
Activity Determination: One unit of EconoTaq DNA Polymerase catalyzes the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 70°C in 50 mM Tris-HCl (pH 9.0), 50 mM NaCl, 5 mM MgCl2, 200 µM dGTP, dATP, dTTP, dCTP (a mix of unlabeled and [33P]dCTP), 10 µg Activated Calf Thymus DNA, and 0.1 mg/ml BSA.
Absence of Endonuclease or Nicking Activity: Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of supercoiled pBR322 DNA for 16 hours at 70°C results in no detectable conversion to relaxed or linear forms detectable by agarose gel electrophoresis.
Absence of Exonuclease Activity: Incubation of 10 U of EconoTaq DNA Polymerase with 1 µg of HindIII-cut lambda DNA for 16 hours at 70°C resulted in no smearing of bands on agarose gels.
Purity: EconoTaq DNA Polymerase is >99% pure as determined by SDS PAGE. There is no detectable DNA contamination.The nucleotides in the Master Mix are certified free of nucleases and phosphatases.
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