The MasterPure™ Complete DNA and RNA Purification Kit enables rapid, high yield purification of high-molecular-weight genomic DNA, total cellular RNA and Total Nucleic Acid (TNA) from many different sample types.
The kit procedure uses a safe, gentle, and scalable salt-precipitation protocol (Figure 1) that eliminates the need for hazardous chemicals and yield-reducing columns. Purification reactions can be scaled up or scaled down depending on the amount of nucleic acid needed and the size of the sample. MasterPure Kits for Nucleic Acid purifications Gram-positive bacteria, yeast DNA and blood samples are also available.
Purification of genomic DNA or total RNA for many applications, including:
Figure 1. Overview of the MasterPure™ Complete Kit protocol.
Figure 2. Comparison of PCR sensitivity using templates obtained using the MasterPure™ Complete DNA and RNA Purification Kit versus a spin-column kit. PCR amplification was performed after extraction from serial dilutions of E. coli cells ranging from 2 × 107 (lanes 1) to 200 (lanes 6). Lane M, DNA marker; lane 7, negative control.
Figure 3. DNA, RNA, and total nucleic acid (TNA) purified from diverse cell sources using the MasterPure™ Complete Kit. M = kb ladder.
Human & mammalian RNA & genomic DNA
Tissue-culture cell lines
Table 1. Examples of targets and samples analyzed using MasterPure™ DNA and RNA Purification Kits. Using an extremely simple salt-precipitation protocol, TNA, DNA, or RNA can be isolated from virtually any source in less than 1 hour.
|Sample||Sample Size||TNA µg||DNA µg||RNA µg|
|HeLa/HL60 cells||1 × 106 cells||10-30||3-12||7-15|
|Buffy coat||300 µl||40-55||40-55||3-6|
|Mouse tail||0.5 cm||25-30||9-11|
|E. coli||3.5 × 106 cells||2.5-2.8||1.3-1.6||1.6-1.8|
|S. mutans||1.5 ml||0.9|
|2.2 × 106 cells||11-18|
|1.1 × 107 cells||70-78|
*For extraction of yeast DNA, the MasterPure™ Yeast DNA Purification Kit is recommended.
Table 2. Example of yields of nucleic acids obtained using MasterPure™ Complete DNA and RNA Purification Kits.
Figure 4. Consistent purification of DNA, TNA, and RNA. Five different genomic DNA (lanes 1-5), TNA (lanes 6-10), and total cellular RNA (lanes 11-15) samples were isolated from frozen bovine liver with the MasterPure™ Complete DNA and RNA Purification Kit. Lane M, Kilobase ladder.