T7 RNA Polymerase catalyzes the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognizes the T7 promoter and terminator sequences with high specificity.
Unit Definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37°C.
Source: Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene.
|Unit Concentration||50,000 U/mL|
|SS Exonuclease||500 U <0.1% released|
|DS Exonuclease||500 U <0.1% released|
|Endonuclease||500 U <10% converted|
|RNAse Contamination||500 U = none detected|
|E. coli 16S rDNA Contamination||500 U <10 copies|