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OverExpress™ C41(DE3) and C43(DE3) Competent Cells: OverExpress C43(DE3) pLysS Chemically Competent Cells

  • Express genes cloned into any T7 vector with these BL21(DE3) derivatives
  • Effective in expressing toxic & membrane proteins
  • Cited in over 350 research articles
Please note: commercial entities require a license from Brookhaven Science Associates to purchase this product.
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Product Description Size Cat. No. Price Quantity BUY
OverExpress C43(DE3) pLysS Chemically Competent Cells  12 rxns (SOLOs) 60448-1 $242.00
   24 rxns (SOLOs) 60448-2 $428.00
ORDER INFORMATION
Each OverExpress kit contains Electrocompetent or Chemically Competent Cells in SOLO packaging (1 transformation per tube), Expression Recovery Medium (lactose minus), pUC19 Positive Control Plasmid, pAVD10 Verification Plasmid, and complete protocols. ComboPacks contain 3 reactions each of C41(DE3), C43(DE3), C41(DE)pLysS, and C43(DE3)pLysS, as either electrocompetent or chemically competent cells, as indicated.

Important Product Use Information
OverExpress Cells are licensed exclusively to Lucigen Corporation by Imaxio S.A. under US Pat. 6,361,966 and others. Purchase of OverExpress Cells is accompanied by a non-exclusive, non-transferable license for research use only. Commercial use requires a separate license available from Imaxio S.A. OverExpress Cells are also sold under a license issued to Lucigen Corporation by Brookhaven Science Associates, LLC (BSA) for research use by noncommercial entities. Commercial entities require a license from BSA even for research use.

OverExpress Genotypes
OverExpress C41(DE3): F – ompT hsdSB (rB- mB-) gal dcm (DE3)
OverExpress C41(DE3)pLysS: F – ompT hsdSB (rB- mB-) gal dcm (DE3) pLysS (CmR)
OverExpress C43(DE3): F – ompT hsdSB (rB- mB-) gal dcm (DE3)
OverExpress C43(DE3)pLysS: F – ompT hsdSB (rB- mB-) gal dcm (DE3) pLysS (CmR)


Table 1. OverExpress Transformation Efficiencies

Electrocompetent Cells
cfu/µg DNA
C41(DE3)
> 1 × 1010
C41(DE3)pLysS
> 1 × 109
C43(DE3)
> 1 × 1010
C43(DE3)pLysS
> 1 × 109
Chemically Competent Cells
C41(DE3)
> 1 × 106
C41(DE3)pLysS
> 1 × 106
C43(DE3)
> 1 × 106
C43(DE3)pLysS
> 1 × 106

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"Your OverExpress C41(DE3) cells express at least 2x the level of other engineered BL21s with my minimally toxic proteins, and many fold higher levels with my more toxic proteins."
- Kevin Glenn, M.D. Assistant Professor of Internal Medicine, University Iowa

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