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OverExpress™ C41(DE3) and C43(DE3) Competent Cells: OverExpress C41(DE3)pLysS Electrocompetent Cells

  • Express genes cloned into any T7 vector with these BL21(DE3) derivatives
  • Effective in expressing toxic & membrane proteins
  • Cited in over 350 research articles
Please note: commercial entities require a license from Brookhaven Science Associates to purchase this product.
  • Specifications

  • Manuals

  • Resources

OverExpress Genotypes
OverExpress C41(DE3): F – ompT hsdSB (rB- mB-) gal dcm (DE3)
OverExpress C41(DE3)pLysS: F – ompT hsdSB (rB- mB-) gal dcm (DE3) pLysS (CmR)
OverExpress C43(DE3): F – ompT hsdSB (rB- mB-) gal dcm (DE3)
OverExpress C43(DE3)pLysS: F – ompT hsdSB (rB- mB-) gal dcm (DE3) pLysS (CmR)


Table 1. OverExpress Transformation Efficiencies

Electrocompetent Cells
cfu/µg DNA
C41(DE3)
> 1 × 1010
C41(DE3)pLysS
> 1 × 109
C43(DE3)
> 1 × 1010
C43(DE3)pLysS
> 1 × 109
Chemically Competent Cells
C41(DE3)
> 1 × 106
C41(DE3)pLysS
> 1 × 106
C43(DE3)
> 1 × 106
C43(DE3)pLysS
> 1 × 106
Document File Name Type

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\"Your OverExpress C41(DE3) cells express at at least 2x the level of other engineered BL21s with my minimally toxic proteins, and many fold higher levels with my more toxic proteins.\"
- Kevin Glenn, M.D. Assistant Professor of Internal Medicine, University Iowa

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