Ribonuclease A (RNase A) is an endoribonuclease that cleaves single-stranded RNA at the 3´ end of pyrimidine residues, forming oligoribonucleotides having 3´-terminal pyrimidine-3´-phosphates. Pyrimidine-3´-monophosphates are also released by RNase A cleavage of adjacent pyrimidine nucleotides. Modified RNA containing pyrimidine-2´-fluoro-dNMPs, such as DuraScript® RNA made by in vitro transcription using the DuraScribe® T7 & SP6 Transcription Kits is completely resistant to cleavage by RNase A.
Composition and Concentration: Provided on the basis of weight at 5 mg/ml in Storage Buffer.
Storage Buffer: 50% glycerol containing 25 mM sodium acetate (pH 4.6).
Quality Control: RNase A is free of contaminating exo- and endodeoxyribonuclease activities.