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RapiDxFire™ Thermostable Reverse Transcriptase

A truly thermostable reverse transcriptase for fast synthesis of short cDNA (< 1 Kb)

  • Extremely active at high temperatures (55 to 80°C): Improves specificity of cDNA Synthesis from diverse RNA templates
  • Sensitive: Detects ≤100 copies of RNA in two-step RT-qPCR assays
  • Short reaction time (5 minutes or less): Streamlined RT-qPCR workflow and faster time to results
  • Stable at room temperature (> 3 months): Simplifies setup on automation decks (no cold storage required) and enables use in environments with limited cold-storage
  • Lyo-compatible: Enzyme formulation is free of glycerol -and other components- that are known to interfere with downstream lyophilization
  • Batch to batch reproducibility: Manufactured in an ISO 13485-certified facility
  • Custom OEM ProductBulk/OEM available: Contact us about bulk orders, custom formulations, and dispensing options.
  • Special Offer* – Receive 20% off your first order by using code 20Rapid during checkout

  • Ordering

  • Specifications

  • Manuals

  • Resources

Product Description Size Cat. No. Price Quantity BUY
RapiDxFire Thermostable Reverse Transcriptase, 3 Units/µL  50 Rxns 30250-1 $350.00
   250 Rxns 30250-2 $1487.00
Contents include RapiDxFire Thermostable Reverse Transcriptase (3 Units/ µL) and 10X RapiDxFire Reaction Buffer. The enzyme pack sizes (50 or 250 Rxns) are based on 25 µL reaction volumes. For sample requests and bulk or custom orders, please visit Custom & OEM Solutions from Lucigen.

* This promotion is only available to US customers purchasing qualifying products in a single purchase order with a minimum of [1] to a maximum of [10] by August 30, 2019. Qualified products are [RapiDxFire Thermostable Reverse Transcriptase Cat No. 30250-1 and 30250-2]. Promo code is required at the time the order is placed for the 20% discount to be applied. Discount valid on list price in effect at the time order is received by LGC and applies to direct orders only. Cannot be combined with other offers or promotions. One time use only. Terms and conditions are subject to change without notice. Offer void where prohibited, licensed or restricted by federal, state, provincial or local laws or regulation or agency/institutional policy.

Legal Information/Updates:
US Patent 8093030 for RapiDxFire™ is owned by Lucigen Corp. For research use only. Not for use in diagnostic procedures.
  • Storage: Store at -20°C.
  • Storage Buffer: RapiDxFire Thermostable RT is supplied in a glycerol-free, Triton X-100-free buffer.
  • Unit Definition: One RT unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into an acid-insoluble form in 10 minutes at 55°C (using poly(rA) • oligo(dT) as template primer and 1X kit -supplied buffer formulation).
  • Functional QC Assay: RapiDxFire Thermostable RT is functionally tested with gene-° primer and bacteriophage MS2 RNA. In this reaction the enzyme converts 1000 copies of MS2 RNA into 520 bp cDNA in 5 minutes at 60°C.
  • Endonuclease: Free of contaminating endonucleases at the 10 Unit level.
  • Exonuclease: Free of contaminating exonucleases at the 10 Unit level.
  • RNase Activity: No RNase activity detected using a fluorescence-based RNase activity assay.
Document File Name Type
Manual RapiDxFire-reverse-transcriptase-manual PDF

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