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Sygnis SunScript™ One Step RT-PCR Kit

A novel high-temperature One Step RT-PCR kit based on the combination of SunScript™ Reverse Transcriptase RNaseH- and high-quality Taq Polymerase for the reverse transcription and PCR amplification of specific RNA fragments in one convenient single step.

  • Improved thermal stability for the reverse transcription step: Works up to 85°C (60-65°C recommended).
  • Transcription and amplification of difficult RNAs: Transcribes RNAs with high GC content or complex secondary structures.
  • Great sensitivity: Starts with picogram amounts of RNA
  • Improved PCR specificity: Hot start Taq polymerase greatly improves specificity during amplification
  • High yields of amplified DNA
  • High reproducibility across reactions: Best choice for processing multiple samples with minimum deviations.
  • Easy handling: Reagents are added in a single step, which also minimizes dangers of contamination and reduces overall handling time.
  • Ordering

  • Specifications

  • Manuals

  • Resources

Product Description Size Cat. No. Price Quantity BUY
Sygnis SunScript™ One Step RT-PCR Kit  25 rxn SYG430025 $210.00
   100 rxn SYG430100 $573.00

Each SunScript™ One Step RT-PCR Kit contains: Enzyme Mix, 2X Reaction Buffer and water. The complete manual is available under the Manual tab. Lucigen is an authorized distributor of Sygnis products in the US.

SunScript™ One Step RT-PCR Kit is intended for molecular biology use only and in vitro use only. This product is not intended for diagnosis, prevention or treatment of a disease in human beings or animals.

Technical Specifications:
The SunScript™ One Step RT-PCR Kit has been tested in the reverse transcription / amplification of a 271 bp fragment of β-actin with a RNA dilution series.

The 2x Reaction Buffer contains a proprietary formulation which is optimized for both the reverse transcription and the PCR reaction and includes dNTPs, MgCl2 and stabilizers (to provide a final concentration of 250 nM of each dNTP and 3.5 mM MgCl2 in the reaction).

Quality Control:
Each batch of SunScript™ One Step RT-PCR Kit is tested against predetermined specifications to ensure consistent product quality.

Shipping and Storage:
This product is shipped in dry ice. Upon receipt, it should be stored immediately at -20°C in a non-frost-free (constant temperature) freezer. If stored correctly, the product can be kept for at least six months after shipping without displaying any reduction in performance. For longer periods of time, store the kit at -80°C.

Document File Name Type
Manual Sygnis-SunScript-One-Step-RT_PCR-kit-Handbook PDF
Document File Name Type
SDS Sygnis-SunScript-One-Step-RT-PCR-Kit-MSDS.pdf PDF

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