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Transposon Mutagenesis

Transposome mutagenesis is an easy and highly effective method for generating bacteria with improved genetics/characteristics, decreased pathogenicity and gene knockouts. The EZ-Tn5™ Transposon and Transposome Kits allow you to mutagenize DNA in vitro or bacterial strains in vivo, respectively. Multiple kits are available for various applications including insertion mutagenesis, promoter insertion and bacterial origin of replication insertion for downstream rescue of your favorite mutants. Choose from the kits below for the application of your choice. You’re only limited by your imagination in what you can do with this powerful technology.

In Vitro Tn5 Transposon Mutagenesis

EZ-Tn5™ <R6Kγori/KAN-2> Insertion Kit
Insert R6K origin for plasmid rescue and DNA sequencing applications.
EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit
Insert Tn7 promoter for gene expression applications.
EZ-Tn5™ <KAN-2>, EZ-Tn5™ <TET-1>, and EZ-Tn5™ <DHFR-1> Insertion Kits
Insert selectable marker for many applications, including DNA sequencing and functional epitope mapping.
EZ-Tn5™ Transposase
Generate EZ-TN5 Transposomes™ for in vitro transposomics.

In Vivo Tn5 Transposon Mutagenesis

EZ-Tn5™ <R6Kγori/KAN-2>Tnp Transposome™ Kit
Insert selectable marker for transposon applications in non-E. coli bacteria.
EZ-Tn5™ <KAN-2>Tnp Transposome™ Kit
Insert selectable marker for 100’s of referenced applications in bacteria, including mutagenesis and characterization of gene function.
TypeOne™ Restriction Inhibitor
Increase plasmid transformation efficiency in bacteria, and insertion efficiency of EZ-Tn5 Transposome complexes.

TransforMax™ EC100D™ pir+ and pir-116 Electrocompetent E. coli

TransforMax™ EC100D™ pir+ and pir-116 Electrocompetent E. coli
Clone large inserts with high efficiency recA, endA strain. Transform by electroporation.

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