Uracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA containing deoxyuridine in place of thymidine. UNG is active on both ssDNA and dsDNA that contain uracil, but has no activity on RNA or 2´-deoxyuridine- 5´-monophosphate. UNG is ideal for studying repair of abasic sites in double-stranded DNA.
Unit Definition: One unit of UNG catalyzes the release of 1 nmol of uracil from uracil-containing DNA in 1 hour at 37°C under standard assay conditions.
Note: Epicentre's unit is 5- to 20-fold more active than a unit as defined by other suppliers. Therefore, Dilution Buffer is provided for applications requiring lower concentrations of enzyme.
Dilution and Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.
Quality Control: UNG is free of detectable exo- and endonuclease and RNase activities.